Published: Dec 24, 2020
  
 
     
 
Keywords:
formalin-fixed paraffin-embedded tissue PCR for M Tuberculosis Ziehl-Neelsen stain acid-fast bacilli sensitivity
  
     
 
   
 
 
 
 
 
 
 
 
 
 
 

 

 
   
 
 
 
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    Home / Archives / Vol. 16 No. 2 (2020): July - December / Research Article  
 
   
 

The study of benefit of an additional Ziehl-Neelsen stain for detection
of acid-fast bacilli in tissue sections
   
 
   
   
     
 
Pakorn Arunsawat
ภาควิชาพยาธิวิทยา คณะแพทยศาสตร์จุฬาลงกรณ์มหาวิทยาลัย

Abstract

Objective: This study was aimed to determine whether an additional Ziehl-Neelsen (ZN) stain section could increase the positive rate.


Materials and Methods:


Formalin-fixed paraffin-embedded (FFPE) tissue with positive polymerase chain reaction (PCR)-based detection of M. tuberculosis were retrieved from the Pathology File at King Chulalongkorn Memorial Hospital from 2016 to 2018. The original ZN stain was reviewed, with an additional ZN stain performed.


Results: Of 78 cases with positive PCR, 15 (19.23%) were positive for acid-fast bacilli (AFB) in the original ZN section. Additional 4 cases (19 positive cases in total) were shown to contain AFB in the additional ZN stain section (sensitivity increased from 19.23% to 24.36%).


Conclusion: An additional ZN stain section increased sensitivity (5.13%) for the detection of AFB in tissue sections.

  
     
     
 
    How to Cite  
     
  Arunsawat, P. (2020). The study of benefit of an additional Ziehl-Neelsen stain for detection of acid-fast bacilli in tissue sections. Journal of Charoenkrung Pracharak Hospital, 16(2), 1–7. Retrieved from https://he02.tci-thaijo.org/index.php/JCP/article/view/241261  
     

 
 
     
     
 
    Issue  
     
  Vol. 16 No. 2 (2020): July - December  

 
 
     
     
 
    Section  
     
  Research  article  

 
 
     
     
     
 

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